mouse muc5ac elisa kit  (Cusabio)

Journal: International Journal of Molecular Sciences

Article Title: Dictamnine Exhibits Anti-Asthmatic Effects by Modulating TGF-β/Smad2/3 Signaling in a Murine Asthma Model and Human Bronchial Epithelial Cells

doi: 10.3390/ijms262411891

Figure Lengend Snippet: Dictamnine reduced Th2 cytokine, MUC5AC, and OVA-specific IgE levels in OVA-induced asthmatic mice. ( a – c ) BALF cytokine levels: ( a ) IL-4, ( b ) IL-5, and ( c ) IL-13. ( d ) MUC5AC levels in BALF. ( e ) OVA-specific IgE levels in serum. Data are expressed as mean ± SEM ( n = 5 per group). * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to the AC group.

Article Snippet: MUC5AC level in BALF was determined using the Mouse MUC5AC ELISA kit (CSB-E11040m; Cusabio, Wuhan, China).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Dictamnine Exhibits Anti-Asthmatic Effects by Modulating TGF-β/Smad2/3 Signaling in a Murine Asthma Model and Human Bronchial Epithelial Cells

doi: 10.3390/ijms262411891

Figure Lengend Snippet: Dictamnine reduced Th2 cytokine, MUC5AC, and OVA-specific IgE levels in OVA-induced asthmatic mice. ( a – c ) BALF cytokine levels: ( a ) IL-4, ( b ) IL-5, and ( c ) IL-13. ( d ) MUC5AC levels in BALF. ( e ) OVA-specific IgE levels in serum. Data are expressed as mean ± SEM ( n = 5 per group). * p < 0.05, ** p < 0.01, and *** p < 0.001 compared to the AC group.

Article Snippet: MUC5AC level in BALF was determined using the Mouse MUC5AC ELISA kit (CSB-E11040m; Cusabio, Wuhan, China).

Techniques:

Journal: Science Advances

Article Title: Bordetellae colonization oligosaccharide (b-Cool), a glycan crucial for nasal colonization

doi: 10.1126/sciadv.adw7764

Figure Lengend Snippet: Although mouse nasal epithelia (MNE) ( A ) and mouse tracheobronchial epithelia (MTE) ( B ) shared similar morphology, MNE secrete more mucus than MTE to the apical surface, as quantified by Muc5Ac ELISA ( C ). To further evaluate the role of mucus in B. bronchiseptica colonization, identical CFUs of wild type or Δ b-Cool were inoculated to MTE, and either control HBSS or mucus was supplemented before inoculation and after each apical wash. Δ b-Cool showed no colonization defect when supplemented with HBSS ( D ) but showed a profound colonization defect when supplemented with MNM ( E ) and PSM ( F ). To further investigate the interaction of wild type or Δ b-Cool with mucus, ovalbumin (OVA), MNE, or PSM was coated to 96-well plates, and identical CFUs of wild type or Δ b-Cool were inoculated to corresponding wells. Plates were incubated at 4° and 37°C for 1 hour, and unbound bacteria were recovered and plated for CFUs. Identical amounts of wild type and Δ b-Cool were recovered from each group at 4°C ( G ). At 37°C, similar amounts of wild type and Δ b-Cool were recovered from OVA-coated wells, while significantly less Δ b-Cool was recovered than the wild type from mucus-coated wells ( H ). Last, wild type or Δ b-Cool was inoculated to C57BL/6 mice (wild-type mice) or mucin-deficient mice (Muc5Ac −/− and Muc5Ac −/− Muc5B −/− ) and bacterial CFUs in the nasal cavity were examined at 3 dpi. The colonization defect of Δ b-Cool was partially rescued in Muc5Ac −/− mice and nearly completely rescued in the mucins double deletion mice, Muc5Ac −/− Muc5B −/− ( I ), indicating that b-Cool provide resistance to both mucins (Muc5Ac and Muc5B). Data at each time point represent four or more biological replicates. All experiments were independently performed two or three times. * P < 0.05 and *** P < 0.001.

Article Snippet: The level of Muc5Ac was quantified by NOVUS mouse Muc5Ac ELISA kit (colorimetric) according to the instruction.

Techniques: Enzyme-linked Immunosorbent Assay, Control, Incubation, Bacteria